clpC [2017-08-02 09:03:33]

AAA unfoldase, ATPase subunit of the ClpC-ClpP protease, directs proteins phosphorylated on arginine residues to ClpP

Locus: BSU00860

Isoelectric point: 5.75

Molecular weight: 89.93 kDa

Protein length: 810 aa

Gene length: 2430 bp

Function: protein degradationpositive regulator of autolysin (LytC and LytD) synthesis

Product: AAA unfoldase, ATPase subunit of the ClpC-ClpP protease

Essential: no

Synonyms: mecB

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall/ other
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Heat shock proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 103,572 → 106,004

Phenotypes of a mutant

• inactivation of clpC reduces sporulation efficiency to 0.4% that of wild type cells; delayed entry into sporulation, defect in engulfment with reduced SigG activity, and production of small spores PubMed

The protein

Catalyzed reaction/ biological activity

• ATPase/chaperone

Protein family

• mecA family (according to Swiss-Prot) clpA/clpB family. ClpC subfamily (according to Swiss-Prot), AAA -type ATPase (IPR013093) InterPro (PF07724) PFAM

Paralogous protein(s)

• ClpE, ClpX

Domains

• AAA-ATPase PFAM

Modification

• phosphorylated on Arg-5 and Arg-254 PubMed

Structure

• 2K77 (N-terminal domain)
• 3PXG, [PDB|3J3U 3J3U] (the MecA-ClpC complex) PubMed

Localization

• cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with ClpP Pubmed
• forms foci coincident with nucleoid edges, usually near cell poles PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: ctsR-mcsA-mcsB-clpC-radA-disA
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed PubMed, in SigB regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • CtsR: repression, PubMed, in CtsR regulon
  • Spx: activation, PubMed, in Spx regulon

  Regulation

  • expressed during germination and spore outgrowth PubMed
  • induction during diamide stress (Spx) PubMed

Biological materials

Mutant

• clpC::tet available from the Hamoen Lab
• BP98 (clpC::spc), available in Fabian Commichau's lab PubMed
• BKE00860 (clpC::erm trpC2) is available from the Bacillus Genetic Stock Center PubMed

GFP fusion

• C-terminal GFP fusions (single copy, also as CFP and YFP variants) available from the Hamoen Lab

Antibody

• available in Ulf Gerth's and Jörg Stülke's labs

Labs working on this gene/protein

• Leendert Hamoen, Newcastle University, UK homepage
• Kürsad Turgay, Freie Universität Berlin, Germany homepage

References

Reviews

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Original Publications

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